Whole Cells

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Biotransformation of Progesterone by Whole Cells of Filamentous Fungi Aspergillus brasiliensis

Microbial steroid biotransformation have found wide-reaching application for the production of more precious and functionalized compounds due to their high regio-and stereoselectivity. In this study, the possibility of using filamentous fungi Aspergillus brasiliensis cells in the biotransformation of progesterone (I), a C-21 steroid hormone was studied for the first time.The fungal strain was i...

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O-38: Concurrent Whole-Genome Haplotyping and Copy-Number Profiling of Single Cells

Background Methods for haplotyping and DNA copynumber typing of single cells are paramount for studying genomic heterogeneity and enabling genetic diagnosis. Before analyzing the DNA of a single cell by microarray or next-generation sequencing, a whole-genome amplification (WGA) process is required, but it substantially distorts the frequency and composition of the cell’s alleles. As a conseque...

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I-44: Concurrent Whole-Genome Haplotyping and Copy-Number Profiling of Single Cells

Background Methods for haplotyping and DNA copynumber typing of single cells are paramount for studying genomic heterogeneity and enabling genetic diagnosis. Before analyzing the DNA of a single cell by microarray or next-generation sequencing, a whole-genome amplification (WGA) process is required, but it substantially distorts the frequency and composition of the cell’s alleles. As a conseque...

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Biotransformation of Progesterone by Whole Cells of Filamentous Fungi Aspergillus brasiliensis

Microbial steroid biotransformation have found wide-reaching application for the production of more precious and functionalized compounds due to their high regio-and stereoselectivity. In this study, the possibility of using filamentous fungi Aspergillus brasiliensis cells in the biotransformation of progesterone (I), a C-21 steroid hormone was studied for the first time.The fungal strain was i...

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Fluorescent - Dna Probes for Whole Cells

Fluorescent-dye-conjugated oligonucleotides were used to classify 14 Fibrobacter strains by fluorescence microscopy. On the basis of partial 16S rRNA sequences of six Fibrobacter strains, four hybridization probes were designed to discriminate between the species Fibrobacter succinogenes and Fibrobacter intestinalis and to identify F. succinogenes subsp. succinogenes. After in situ hybridizatio...

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ژورنال

عنوان ژورنال: Nature

سال: 1968

ISSN: 0028-0836,1476-4687

DOI: 10.1038/2201006b0